Abstract
Genes encoding proteins with PHD (plant homeodomain) finger motif (C4HC3) are highly conserved fromArabidopis toHomo sapiens. One of the major functions of these genes is regulating the expression of homeotic genes during the stage of embryonic development. They play a role in cell-cycle and cell differentiation and seem to be related with some human malignant diseases, such as leukemia. A human placenta cDNA library has been screened with cDNA probe amplified by PCR. The PCR primers have been designed according to theM96 (a mouse gene encoding a protein with PHD domain) homologous data in dbEST. A 2.1 kb insert fragment in a positive cDNA clone has been isolated and sequenced. This new full-length cDNA is namedPHF2 (GenBank Acc: AF052205). The putative protein composed of 567 aa has two typical PHD fingers at its N-terminus. Meanwhile it is identified that there are several alternatively spliced transcripts ofPHF2 in different human tissues through the PCR amplification, Northern blot hybridization and analysis of genomic DNA structure.
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