Abstract
KCS10 gene is one of the members of the β-ketoacyl-CoA synthase(β-ketoacyl coa synthase,KCS) gene family.To understand the function of KCS10 gene,genome walking and RACE techniques were used to clone BnKCS10 gene from B.napus cv.Zhongshuang 9.The BnKCS10 gene has a 2 344bp-coding region containing 2 introns spanning 527bp and 167bp respectively.BnKCS10 gene expressed at significantly higher levels in flower buds,and also in stigma and 30d developing seeds than in the vegetative organs.The yeast cells tranformed with BnKCS10 gene produced KCS10 protein normally,but no synthesis of very long chain fatty acids was detected.
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