Abstract
The ZmRXO1 gene is a nucleotide-binding site leucine-rich repeat (NBS–LRR) type of R gene in maize (Zea mays). To understand the regulatory mechanism of ZmRXO1 gene expression, we isolated and characterized the ZmRXO1 promoter (PZmRXO1)—the 5′ flanking region of ZmRXO1. A series of PZmRXO1 deletion derivatives, R1–R4, from the translation start code (−1,576, −934, −829, and −582) were fused to the GUS reporter gene, and each deletion construct was analyzed by Agrobacterium-mediated transformation into tobacco. Sequence analysis showed that several cis-acting elements (MBS, Box-I, TGA-element and CCAAT-box) were located within the promoter. Deletion analysis of the promoter suggested that the 1,576-bp fragment upstream of ZmRXO1 gene showed a high level of GUS expression in tobacco. The promoter sequence (−582 to −1) was sufficient to improve transcription of GUS gene under hormones (MeJA, GA, ABA), drought and low temperature. Moreover, there might be repressor elements in the region (−1,576 to −934 bp) to repress ZmRXO1 gene expression under treatment with salicylic acid.
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