Abstract
The Δ 9 -stearoyl-acyl carrier protein desaturase (SAD) plays key roles in determining the ratio of saturated to unsatu- rated fatty acids in higher plants. The 720 bp 5' flanking sequence of peanut SAD (AhSAD) gene was isolated from the genomic DNA of peanut cultivar Yuhua 9326 by nested PCR using genomic walking method. The fragment of 5' UTR was obtained using 5' RACE (Rapid Amplification of cDNA End), confirming that the 720 bp 5' flanking sequence is promoter fragment based on sequence alignments. Bioinformatics analysis indicated that AhSAD promoter contained several light, hormone responsive ele- ments and enhancer-like elements as well as CAAT box and TATA box. To study the function of this promoter, we constructed a binary expression vector pBI-PAhSAD by replacing CaMV35S promoter of pBI121 with the AhSAD promoter, which was intro- duced into Arabidopsis and transiently expressed in peanut, respectively, by Agrobacterium-mediated transformation. Histo- chemical staining analysis showed that the GUS gene mainly expressed in roots, stem, leaves, cotyledon and peanut pegs. The histochemical staining was observed in peanut whole leaves, while only in Arabidopsis veins of cauline leaves.
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