Cloning and expression regularity of TdTrx1 and TdTrx17 in Tapes dorsatus under Vibrio parahaemolyticus stress

Abstract

Tapes dorsatus are common economic shellfish in the Beibu Gulf and are often exposed to silt containing a large number of bacteria. Vibrio parahemolyticus is a common pathogenic bacterium in the marine environment, which is a potential threat to the health of marine organisms. In this study, the thioredoxin 1 (TdTrx1) and thioredoxin 17 (TdTrx17) genes were cloned by RT-PCR, and their expression changes in the gills and visceral mass tissues of T. dorsatus were studied using qRT-PCR under the stress of Vibrio parahemolyticus. The results showed that the coding sequences of TdTrx1 and TdTrx17 genes were 1045 bp and 425 bp, respectively. qRT-PCR analysis showed that TdTrx1 and TdTrx17 were expressed in 7 tissues, and that expression was highest in the gills, at 23.9 and 25.5 times the expression in the foot, respectively. Under Vibrio parahaemolyticus stress, the highest expression of TdTrx1 at 12 h was 310 times that at 72 h, and the highest expression of TdTrx17 at 9 h was 7.5 times that at 72 h. In this study, TdTrx1 and TdTrx17 genes were cloned for the first time, which were highly expressed in the visceral mass tissues. The expression of the TdTrx1 and TdTrx17 genes changed significantly after Vibrio parahemolyticus stress, indicating that both the TdTrx1 and TdTrx17 genes were involved in the resistance process of Vibrio.