Abstract
B18 is an immunoglobulin superfamily glycoprotein produced by vaccinia virus which resembles to interferon alpha receptors. It acts both in solution and also when associated with the cell surface. This B18 protein was expressed in heterologous host and purified based on affinity column chromatography. The purified B18 protein has been evaluated for biological activity in Pig Kidney- 15 cells (PK-15) which has been reported to have α –interferon receptors and signaling mechanism. The CPE was more than 90% in the presence of protein and around 50% in the absence. B-18 can enhance the infectivity of FMDV to cells having α –interferon receptors. This provides the basis for developing new strategies for improving attenuated vaccines and its capacity to act as an enhancer protein.
Highlights
Attenuated FMDV produced by deleting Lb-protease gene has shown to inhibit host translation mechanism at the fist instant by cleaving Initiation factor E needed for cap dependent translation of the host and thereby allowing the expression of virus specific genes
B18 is an immunoglobulin superfamily glycoprotein produced by vaccinia virus which resembles to interferon alpha receptors
B18 is an immunoglobulin superfamily glycoprotein which resembles to interferon alpha receptors
Summary
Attenuated FMDV produced by deleting Lb-protease gene has shown to inhibit host translation mechanism at the fist instant by cleaving Initiation factor E (eIF-E) needed for cap dependent translation of the host and thereby allowing the expression of virus specific genes. It may be necessary to substitute the lost biological activity of L –protease in L-deleted virus for high titred attenuate virus production for using as vaccine To achieve this we have identified vaccinia enhancer protein (B18) as the substitute for L protease effect. To evade the antiviral effects, VacV produces soluble interferon receptors and proteins that block the activities of interferon. These proteins are directed towards binding and disrupting the function of complement system, cytokines, chemokines and interferons [9,10,11,12]. Vaccinia expresses other proteins that are non-essential for viral replication but promotes virulence by inhibiting components of host immune response to infection, these five vaccinia virus Bcl-2 family proteins (N1, B14, A52, F1 and K7) inhibits the activation of interferon regulatory factor (IRF)3(13,14). Here we expressed B18 protein in heterologous host and the purified protein was used to enhance the infectivity of attenuated Foot-and-Mouth-Disease Virus
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