Abstract
Objective To clone and express truncated hepatitis C virus (HCV) core gene for preparing antigens used for production of HCV-core antibody.Methods HCV-core polypeptide with predominant antigenic determinants was selected by software analysis.The corresponding DNA sequence was amplified by reverse transcription PCR.Then,the gene was cloned into a prokaryotic expression vector for expressing HCV-core antigen.The expressed antigen was purified and detected by indirect ELISA.Results A target gene with correct DNA sequence was obtained.The expressed antigen was a soluble protein and purified for detection of different samples.The results showed that the average absorbance (A) value of HCV-negative samples was 0.081,ratio of A values for HCV-positive samples to negative samples were ＞ 2.1,and A values of hepatitis B-and syphilis-positive samples were ＜ 0.101.Conclusions HCV-core gene is successfully cloned.The expressed HCV core polypeptide has good antigenicity. Key words: Hepacivirus; Gene cloning; Gene expression; Protein purification
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
