Abstract
Background Dengue virus nonstructural protein 1 (NS1) is a highly conserved glycoprotein involved in the production of infectious virus and the pathogenesis of dengue disease [1]. Serum or plasma DENV NS1 level has been found to correlate with viremia titer and disease severity. It can be found in the peripheral blood circulation for up to 9 days from illness onset, but can persist for up to 18 days from illness onset in some cases. Thus NS1 detection offers a larger window of opportunity for diagnosis of dengue compared with virus isolation, RT-PCR or NASBA [2]. In this context, the aim of this work has been the establishment of conditions for expression and purification of the recombinant protein NS1 of dengue virus serotype 2 produced in E. coli for further development of a serological diagnostic method at low cost.
Highlights
Dengue virus nonstructural protein 1 (NS1) is a highly conserved glycoprotein involved in the production of infectious virus and the pathogenesis of dengue disease [1]
The gene encoding the NS1 protein was amplified through RT-PCR and subcloned into the expression vector pET-28a for expression of the recombinant protein
The corresponding recombinant plasmid was transformed into the NS1 protein in bacteria E. coli strain BL21 (DE3) and the clones obtained were expanded and induced with different concentrations of IPTG at 37°C for analysis of the expression of recombinant proteins
Summary
Dengue virus nonstructural protein 1 (NS1) is a highly conserved glycoprotein involved in the production of infectious virus and the pathogenesis of dengue disease [1].
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