Abstract
We have screened genes predominantly expressed in the rat spinal cord, and we report here cloning of the most abundant unknown gene. It is a novel member of the central nervous system (CNS) myelin-constituting proteins, myelin-associated oligodendrocytic basic protein (MOBP). MOBP is abundantly expressed specifically in oligodendrocytes at the mRNA level only next to myelin basic protein (MBP) and proteolipid protein. Two isoforms have been confirmed. One of them has proline-rich tandem repeats and has 84% homology with human counterpart in terms of predicted amino acid sequences. The cDNA-derived primary structure predicts a small, soluble, basic protein. The immunoelectron microscopy has shown that MOBP is expressed throughout compact myelin. All these characteristics are similar to MBP. One definite difference between MBP and MOBP is that MOBP is expressed exclusively in the CNS myelin. These findings suggest that MOBP shares some important functions with MBP in the CNS myelin such as myelin compaction.
Highlights
myelin basic protein (MBP) is a hydrophilic, MBP and myelin-associated oligodendrocytic basic protein (MOBP) is that MOBP is expressed exclusively extrinsic membrane protein with isoelectric points highthear n in the central nervous system (CNS) myelin
Library by modified differential hybridizati(6o1n, a n d we report here the molecular cloning and expression of the most abundant unknown gene expressed in oligodendrocytes. This gene, which we have named myelin-associated oligodendrocytic basic protein (MOBP)h,as the characteristics common with MBP mentioned above, and we propotsheat MOBP has a special function in the CNS myelinthat is shared with MBP
We report here a novel member of the CNS myelin, which shares several characteristics with MBR both are small, I
Summary
(Received for publication, March 17, 1994, and in revised form, August 30, 1994). Yoichi Yamamoto, Ryuzo Mizuno, Tomoya Nishimura, Yasuko Ogawa, Hiroo Yoshikawa, Harutoshi Fujimura, Eijiro AdachiS, Tadamitsu KishimotoO, Takehiko Yanagihara, and Saburo Sakodan. The myelinforming cells are oligodendrocytes i n the central nervous system (CNS)’ and Schwann cells in the peripheral nervous sysmature death.CNS myelin is largely absent, and mostof MBP gene is deleted in the homozygousshiverer mutant (5) Together with those findings, MBP is widely believed to have a function compacting myelin. We screened -3000 plaques from the rat spinal cord cDNA library by modified differential hybridizati(6o1n, a n d we report here the molecular cloning and expression of the most abundant unknown gene expressed in oligodendrocytes. This gene, which we have named myelin-associated oligodendrocytic basic protein (MOBP)h,as the characteristics common with MBP mentioned above, and we propotsheat MOBP has a special function in the CNS myelinthat is shared with MBP
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
