Abstract
The TCP transcription factor family genes are involved in multiple regulation of plant growth and environmental stress. However, these family genes are rarely studied in cassava (Manihot esculenta). There are 36 TCP genes in cassava. Phylogenetic tree analysis indicates that MeTCP can be divided into 8 subgroups. MeTCP4 is one of 36 genes in cassava TCP family, which can be transcribed by miR319 and sheared. MeTCP4 contained an open reading frame (ORF) of 1 269 bp, which encoded 422 amino acids with a predicted molecular weight of 45.75 kD and theoretical PI of 6.17. The full-length cDNA sequence of MeTCP4 was cloned from the cassava genome. The results of quantitative RT-PCR showed that the expression of MeTCP4 can be found in all the tested tissues, with the lowest in roots, second in stems and the highest in leaves. MeTCP4 gene was repressed by drought and low temperature stress. Construction of high and low plant expression vector would provide basis for further study of the function of the gene.
Highlights
Cassava (Manihot esculenta) is a perennial shrub, one of the six major cash crops in the world
From the perspective of TCP transcription factors, this study aims to reveal the molecular mechanism of cassava to respond to drought and low temperature stress
1.1 Family bioinformatics analysis In order to study the evolutionary relationship of MeTCP family members in cassava, the phylogenetic tree of 36 TCP family genes were constructed by Neighbor-joining of MEGA7.0 software
Summary
Cassava (Manihot esculenta) is a perennial shrub, one of the six major cash crops in the world. It is widely cultivated in tropical and subtropical regions of Asia, Africa and Latin America, and eaten by more than 5 billion people (El-Sharkawy, 2004). The research pointed out that cassava (Manihot esculenta) has relative advantages in biological productivity compared with the main tropical food and energy crops. Cassava (Manihot esculenta) has a wide range of rooting systems, and its stomata are highly sensitive to atmospheric humidity and soil water shortage. These characteristics are the physiological basis of drought tolerance of cassava (El-Sharkawy, 1993). It is necessary to explore the characteristics of cassava from the genetic level
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