Abstract
PISTILLATA (PI)-like genes are crucial regulators of flowering in angiosperms. A homologue of PI, designated as AcPI (Genbank accession number HQ717796), was isolated from pineapple cultivar Comte de Paris by reverse transcriptase polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE). The cDNA sequence of AcPI is 907 bp in length and contains an open reading frame of 594 bp, which encodes a protein of 197 amino acids. The molecular weight was 2.29 kDa and the isoelectric point was 9.28. The alignment showed that AcPI had a high identity with CsPIC2 (78.6%), AoPI (77.4%), OrcPI (75.7%) and HPI2 (72.4%). Quantitative real-time polymerase chain reaction (qRT-PCR) analyses in different tissues showed that the expression pattern of AcPI was different from the B-class genes in eudicots. AcPI was expressed in all the tissues investigated. The expression level was very low in fruit stems, bracts, leaves and sepals, high in petals and carpels, and moderate in apical meristems, flesh and stamens. The qRT-PCR analyses in different stages indicated that the expression of AcPI reached the highest level at 40 days after flower inducement, when the multiple fruit and floral organs were forming. It proved the important role of AcPI in floral organs and fruit development. The 35S::AcPI transgenic Arabidopsis plants flowered earlier and had more inflorescences or branches than wild type plants.
Highlights
Most angiosperm flowers, including those of pineapple, are made up of four types of organs that are arranged in concentric whorls from outside to inside: sepals, petals, stamens, and the inner carpels [1].According to the widely accepted ABC model [2,3] of floral organ development, there are three classes of homeotic genes: A-class, B-class and C-class [4,5,6]
AcPI cDNA is 907 bp in length and contains an open reading frame of 594 bp, which encodes a protein of 197 amino acids
The results showed the important role of AcPI in fruit and floral organs development and indicated that ethephon may stimulate the expression of AcPI
Summary
Most angiosperm flowers, including those of pineapple, are made up of four types of organs that are arranged in concentric whorls from outside to inside: sepals, petals, stamens, and the inner carpels [1].According to the widely accepted ABC model [2,3] of floral organ development, there are three classes of homeotic genes: A-class, B-class and C-class [4,5,6]. Other angiosperms and gymnosperm further possess a sister clade of B genes, termed Bsister genes, and expression studies revealed that these genes are predominantly expressed in carpels and ovules [9]. Mutations in these genes cause homeotic conversion of petals in the second whorl to sepals and of stamens in the third whorl to carpels [10,11]. MADS-box is a recognized type of DNA-binding protein [4] including around 60 amino acids, that are highly conserved across developmental control genes from yeast, animals and plants [12,13,14]
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