Cloning and DNA Sequence Analysis of the Haloalkanoic Permease Uptake Gene from Rhizobium sp. RC1

Abstract

The aims of the study were to clone and identify the putative haloacid permease gene in Rhizobium sp. RC1. The putative dehrP gene encoding an uptake protein in Rhizobium sp. RC1 was identified by DNA sequence analysis. An approximately 3.8 kb DNA sequence upstream of dehalogenase D (dehD) in plasmid pSC1 was analyzed and revealed an open reading frame of 1239 kb which encoded for 412 amino acids with calculated subunit molecular weight of 45 kDa and isoelectric point of 9.78. The amino acid sequence of DehrP gave high sequence identity of 86% with putative monochloropropionic acid permease from Agrobacterium sp. NHG3 and 62% with haloacid-specific transporter from Burkholderia cepacia MBA4. Comparison of the predicted amino acid sequence with the CD server (www.ncbi.nlm.nih.gov) NCBI database also revealed the putative DehrP contained signatures of sugar transport proteins of an integral membrane protein. Therefore, a new Rhizobial dehalogenase genetic organization was proposed. However, further characterization of this transporter protein is required to fully comprehend the dehalogenase uptake system of Rhizobium sp. RC1. © 2010 Asian Network for Scientific Information.