Abstract
The cDNAs encoding the porcine luteinizing hormone (LH) β subunit (pLHβ) were isolated from a cDNA library of porcine anterior pituitary constructed in an expression vector λgt11 using anti-pituitary glycoprotein hormone antisera. The nucleotide sequence of pLHβ subunit cDNA clone (543 bases) was determined. The cDNA encodes a signal sequence (20 amino acids) and a further 121 amino acids corresponding to the mature LHβ molecule. The predicted protein sequence differs from that determined by direct peptide sequencing at 6 residues and additionally shows an extended carboxyl terminus. The 5′ untranslated sequence shows a low homology and is extremely long when compared with other mammalian LHβ subunit cDNAs. Northern analysis showed that the length of the pLHβ subunit mRNA is about 0.85 kb.
Published Version
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