Abstract

ince genes of pheromone receptors with obvious conserved regions control the sexual process of fungi, it is of significance to study the conserved property and diversity for the systematic evolution of fungi. In this study, 51 gene segments of pheromone receptor were amplified from 16 wild strains of Agrocybe salicacola and A. aegerita using degenerate primers.For each strain, one to six segments were cloned, with all the nucleic sequences classified into two clusters in Neighborjoining tree. Some sequences of the same species formed a clade, but most sequences were randomly distributed with plentiful diversity. The amino acid sequences translated from the pheromone receptor gene segments were mainly divided into three groups, showing highly conservativity, exposing differences between the two tested mushroom species. Among these amino acid sequences, most segments from different strains of A. aegerita were obviously clustered in minimum-evolution tree, and some sequences from the strains collected in the same areas had high similarity. The NJ and ME trees revealed that pheromone receptors of Agrocybe have two origins. These results provide valuable information for understanding the mating genes or genetic evolution of fungi.

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