Abstract
In this study, two chlorophyll A/B binding protein (CAB) genes (CsCP1 and CsCP2) in tea plant were cloned. The proteins encoded by these genes belong to the external or internal antenna proteins of PS II, respectively. They may be the targets of physiological regulation for tea leaf cell PS II because they all contain multiple functional domains and modifiable sites. The CAB gene family in the tea genome consists of 25 homologous genes. We measured the expression patterns of ten genes in the CsCP1 and CsCP2 subfamily under six different stresses. CsCP1 expression was inhibited in response to 6 kinds of stress; CsCP2 expression was slightly upregulated only after cold stress and ABA treatment. However, the expression levels of CSA016997 and CSA030476 were upregulated significantly in the six stresses. The results suggested that the 10 CAB genes may have different functions in tea leaves. Moreover, changes in the expression of the 10 genes under stress appear to be related to ABA- and MeJA-dependent signalling pathways, and their responses to MeJA treatment is faster than those to ABA. In addition, we introduced our experiences for cloning the genes in the context of complex genomes.
Highlights
In this study, two chlorophyll A/B binding protein (CAB) genes (CsCP1 and CsCP2) in tea plant were cloned
The similarity region identity between CsCP1 and CSA004532 (972 bp), which was a gene from the C. sinensis genome database, is 703/762 (92%), and a gap (30 bp) is noted
As evergreen plant, are subjected to heat from strong light in summer and cold with excess light in winter, so their leaves represent an ideal material for studying photosynthesis mechanisms during cold/hot temperatures with strong light stress
Summary
Two chlorophyll A/B binding protein (CAB) genes (CsCP1 and CsCP2) in tea plant were cloned. The two photosystems in the thylakoid sub-domains of higher plants are comprised of different antenna proteins, the CAB proteins in PS II have attracted considerable attention gevin their complex physiological functions[4,9]. Study on the genes encoding chloroplast proteins in evergreen woody plants and their expression patterns in various circumstances are important to understand the photosynthesis-mechanism changes in different seasons. Sun et al.[15] and Wu et al.[16] compared separately the transcriptome changes in ‘Zijuan’ or ‘Baijiguan’ with other tea cultivars’ during different light and temperature treatments, the relationship between tea phenotypes of the purple or yellow leaves and CAB genes were not mentioned in the two papers because the CAB genes were not cloned. This study can provide some information to further explain the functional characteristics and photosynthesis mechanism of CAB genes in tea plants
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