Abstract
The gene for LPTS/PinX1 encodes a potent telomerase inhibitor and suppresses tumor cell growth. In order to investigate the transcriptional regulation of this gene, we isolated its 5′-flanking region from the human genomic BAC clone and identified a major transcriptional initiation site. The sequence of the 5′-flanking region is GC-rich, lacks canonical TATA box, but contains potential binding sites for a variety of transcription factors. The deletion analysis indicated that the proximal 100 bp (from nt −66 to +34) is essential for minimal promoter activity and the regions of promoter from nt −1272 to −573 and nt −330 to −66 are required for maximal expression of the LPTS/PinX1 gene. Four DNase I hypersensitive sites (DHS1–4) mapping to the regions of transcription initiation and promoter in LPTS/Pinx1 gene were also revealed.
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