Cloning and characterization of the hsp70 multigene family from silver sea bream: Modulated gene expression between warm and cold temperature acclimation

Abstract

The genes encoding heat shock cognate 70 ( hsc70) and inducible heat shock protein 70 ( hsp70) were cloned and characterized from silver sea bream liver. Upon acute heat shock (+7 °C), the transcript abundance of hsc70 was increased 1.7-fold whereas the transcript abundance of hsp70 increased 6.7-fold. The chronic acclimation of sea bream to cold temperature (12 °C) resulted in a downregulation of hsc70 and an upregulation of hsp70 in comparison to levels in sea bream kept at a warmer temperature (25 °C). The expression of heat shock transcription factor I was also increased during cold temperature acclimation. Increased amounts of hepatic insulin-like growth factor 1 transcript, serum thyroxine (T 4), and triiodothyronine (T 3) were also found during cold temperature acclimation whereas serum cortisol remained unchanged. The results from this study demonstrate how temperature acclimation, in fish, can affect the regulation of the hsp70 multigene family and hormonal factors that are associated with anabolism.