Abstract
In this study,we cloned and characterized a geranylgeranyl pyrophosphate synthase(GGPS) gene from Ginkgo biloba Linn.The full-length cDNA sequence of GbGGPS was 1 641 bp containing an open reading frame(ORF) of 1 176 bp,which encoded a 391 amino acids with a predicted molecular mass of 42.51 ku and the theoretical isoelectric point(PI) of 5.98.GbGGPS was an intro-free gene,and its deduced polypeptide contained a chloroplast-targeting signal peptide of 79 amino acids in the N terminal.The secondary structure of GbGGPS was mainly composed of alpha helix and random coil.Comparative analysis showed that GbGGPS had a high similarity to other plant GGPS proteins,and contained all the five conserved domains and functional aspartate-rich motifs of the polyprenyl synthetase family.The homology-based structural modeling showed that GbGGPS has the typical structure of Menthax piperita GGPS.Phylogenetic tree revealed that GbGGPS,TcGGDS,PaGGDS5 and AgGGDS were assigned to the same clade.
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