Abstract
Haemophilus influenzae is a common organism of the human upper respiratory tract; this bacterium is responsible of a wide spectrum for respiratory infections and can generate invasive diseases such as meningitis and septicemia. These infections are associated with H. influenzae encapsulated serotype b. However, the incidence of invasive disease caused by nontypeable H. influenzae (NTHi) has increased in the post-H. influenzae serotype b (Hib) vaccine era. Currently, an effective vaccine against NTHi is not available; due to this, it is important to find an antigen capable to confer protection against NTHi infection. In this study, 10 linear B cell epitopes and 13 CTL epitopes and a putative plasminogen-binding motif (252FYNKENGMY260) and the presence of enolase on the surface of different strains of H. influenzae were identified in the enolase sequence of H. influenzae. Both in silico and experimental results showed that recombinant enolase from H. influenzae is immunogenic that could induce a humoral immune response; this was observed mediating the generation of specific polyclonal antibodies anti-rNTHiENO that recognize typeable and nontypeable H. influenzae strains. The immunogenic properties and the superficial localization of enolase in H. influenzae, important characteristics to be considered as a new candidate for the development of a vaccine, were demonstrated.
Highlights
Haemophilus influenzae is a microorganism that commonly colonizes the upper respiratory tract of both children and adults [1, 2]
There have been reported cases of invasive disease caused by nontypeable H. influenzae (NTHi), and these bacteria have an important role in exacerbation of chronic obstructive pulmonary disease (COPD) in adults; due to this, NTHi is considered as an emerging pathogen [7, 8]
From purified DNA genomic of several H. influenzae strains typeable and nontypeable, the encoding region to enolase of NTHiBUAP96 was ligated to the pRSET-A vector
Summary
Haemophilus influenzae is a microorganism that commonly colonizes the upper respiratory tract of both children and adults [1, 2]. This pathogen causes several types of diseases ranging from respiratory tract infections to severe invasive disease, such as meningitis, sepsis, bacteremia, pneumonia, and epiglottitis [3]. NTHi was the most frequently isolated bacterial pathogens in noninvasive diseases as otitis media and sinusitis in children [1, 5, 6]. A big problem to develop a vaccine is the diversity of NTHi clinical isolates [9], so the challenge is to find a common antigen to all NTHi strains to begin with the development of a vaccine
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