Abstract
We have cloned a full-length cDNA coding for activin β A subunit from a goldfish brain and pituitary cDNA library, which represents the first for activin β A in fish. Sequence analysis of goldfish activin β A shows that this peptide is highly conserved across vertebrates. The mature region of goldfish activin β A shares 81% amino acid identity with that of humans. Messenger RNA of goldfish activin β A is expressed in a variety of tissues including ovary, testis, brain and liver, suggesting a wide range of physiological roles for activin A in the goldfish. The identity of the cloned goldfish activin β A was confirmed by expressing the protein in the Chinese hamster ovary (CHO) cells followed by detection of the specific activin activity in the culture medium using erythroid differentiation factor (EDF) assay with F5-5 cells. Stable CHO cell lines producing high level of recombinant goldfish activin A were established and characterized.
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