Cloning and characterization of defense-related genes from Populus szechuanica infected with rust fungus Melampsora larici-populina.

Abstract

Characterization of defense-related genes is critical for breeding disease-resistant poplar varieties and for better management and control of leaf rust disease. In the present study, full-length cDNAs of five Populus szechuanica defense-related (PsDR) genes, pathogen-related protein 1 (PsPR1), β-1,3-glucanase (PsGns), thaumatin-like protein 1 (PsTLP1), thaumatin-like protein 2 (PsTLP2), and phenylalanine ammonia-lyase (PsPAL), were cloned from the leaves of P. szechuanica infected with Melampsora larici-populina (MLP). PsPR1 (728 bp), PsGns (1189 bp), PsTLP1 (929 bp), PsTLP2 (885 bp), and PsPAL (2586 bp) were predicted to encode 161, 347, 245, 225, and 711 amino acid residue-containing proteins with isoelectric points of 8.53, 4.96, 4.51, 7.32, and 5.87, respectively. Moreover, the deduced PsDR proteins displayed more than 90% similarity to proteins from other Populus species. In response to the avirulent isolate, Sb052, and the virulent isolate, Th053, of MLP, the expression of PsDR genes was rapidly up-regulated in the leaves of P. szechuanica, peaked at 2 or 7 days post-inoculation (dpi), with levels in the incompatible interaction being higher than those in the compatible interaction. Meanwhile, the expression of PsDR genes (except for PsGns) was also differentially up-regulated at 3, 7, or 18 dpi in the petioles of the infected leaves, leaves next to the inoculated leaves, and in the top buds of the infected plants, respectively, compared to that at 0 dpi. These results suggest that these PsDR genes could play distinctive roles in the defense response of poplar against rust infection.