Abstract
The gene and cDNA ( TUB1) encoding an α-tubulin (α-Tub) from Pneumocystis carinii carried in rats were isolated and sequenced. The gene produced a 1.4-kb transcript that contained an open reading frame of 1347 nucleotides coding for a protein of 50326 Da. The nt sequence of TUB1 of P. carinii was 65–71% identical to other TUB1 genes, including those of yeast and human. The deduced amino acid sequence of P. carinii α-Tub showed 75–84% identity to other α-Tubs. Eight small introns resided within TUB1. P. carinii TUB1 was mapped to a 425-kb chromosome. The cloned gene appears to be the only α-Tub-encoding gene in the P. carinii genome.
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