Abstract
An actin encoding gene was cloned by using RT-PCR, 3′ RACE and 5′ RACE from abalone Haliotis discus hannai. The full length of the gene is 1532 base pairs, which contains a long 3′ untranslated region of 307 base pairs and 79 base pairs of 5′ untranslated sequence. The open reading frame encodes 376 amino acid residues. Sequence comparison with those of human and other mollusks showed high conservation among species at amino acid level. The identities was 96%, 97% and 96% respectively compared with Aplysia californica, Biomphalaria glabrata and Homo sapience β-actin. It is also indicated that this actin is more similar to the human cytoplasmic actin (β-actin) than to human muscle actin.
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