Abstract
The nucleotide-binding site (NBS)-Leucine-rich repeat (LRR) gene family accounts for the largest number of known disease resistance genes, and is one of the largest gene families in plant genomes. In the present study, based on the NBS domain, resistance gene analogues (RGAs) have been isolated from peanut, which named PnAG 3 . A full-length cDNA, PnAG 3 was obtained by rapid amplification of cDNA ends (RACE) method. Sequence analysis indicated that the length of PnAG 3 was 1 882 bp, including a complete open reading frame of 1 335 bp encoding PnAG 3 protein of 444 amino acids. Multiple analysis showed that it had a certain homology with known resistance proteins, among which Arachis cardenasii resistance protein had the highest homology (48.01%). The polypeptide has a typical structure of nonTIR-NBS-LRR genes. Real-time fluorescence quantitative PCR analysis showed that after A. flavus infection, the expression of PnAG 3 gene in J11 ( A. flavus resistance species) has increased 16.68, 11.16 and 25.96 times in seed coat, kernel and pericarp, respectively. But it only increased 2-3 times in JH1012 ( A. flavus sensitive species). The cloning of putative resistance gene from peanut provides a basis for studying the structure and function of peanut disease-resistance relating genes and disease resistant genetic breeding in peanut.
Highlights
Peanut (Arachis hypogaea L.) is one of the four major oil crops in the world
The nucleotide-binding site (NBS)-Leucine-rich repeat (LRR) gene family accounts for the largest number of known disease resistance genes, and is one of the largest gene families in plant genomes
The polypeptide has a typical structure of nonTIR-NBS-LRR genes
Summary
Peanut (Arachis hypogaea L.) is one of the four major oil crops in the world. Peanut has been the highest export crop in china. Peanut is susceptible to A. flavus infect and pests which cause yield reduction and quality decline problems, especially by the aflatoxin contamination. Peanut A. flavus infection and its subsequent accumulation of toxic and carcinogenic secondary metabolites, aflatoxin, are serious agricultural problems, especially in dry conditions. The European Union (EU), Association of Southeast Asian Nations (ASEAN), Japan and other importing countries have made standards of peanut aflatoxin and pesticide residues more stringent and peanut production areas of aflatoxin contamination can not be resolved a long-term, there are excessive of aflatoxin in peanut, which had been a limiting factor of our peanut exports. Studies on resistant varieties of peanut should improve the understanding of resistance mechanisms to A. flavus and cultivating A. flavus resistance peanut varieties
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