Abstract
A homologue of the mammalian translationally controlled tumor protein (TCTP) was cloned from the human parasite Schistosoma mansoni (SmTCTP). Sequence analysis showed that SmTCTP differed from other reported TCTPs in having only one signature sequence. Subsequently, SmTCTP was cloned in a T7 expression system and expressed as a histidine-tagged fusion protein. Recombinant SmTCTP (rSmTCTP) has a molecular mass of approximately 23 kDa with the histidine tag. Further analysis showed that SmTCTP transcripts and protein are expressed in all life cycle stages of the parasite within the vertebrate hosts. Interestingly, antibodies to SmTCTP were present in the sera of mice 9 weeks after infection with S. mansoni. Characterization studies showed that rSmTCTP is a calcium-binding protein that can cause histamine release from basophil/mast cells and induce eosinophil infiltration. These findings suggest that SmTCTP may have an important role in the development of allergic inflammatory responses associated with schistosomiasis and may be a target for new drug development.
Highlights
A family of translationally controlled tumor proteins (TCTP)1 was initially demonstrated in the growth phase of tumor cells [1]
Isolation and Sequence Analysis of SmTCTP—A cDNA fragment of 513 bp was isolated from S. mansoni cercarial cDNA using primers derived from S. japonicum TCTP sequence and EST sequences of S. mansoni as described under “Experimental Procedures.”
SmTCTP is the first recombinant protein characterized from S. mansoni that has a histamine-releasing function
Summary
The nucleotide sequence(s) reported in this paper has been submitted to the GenBankTM/EBI Data Bank with accession number(s)AF358139. The filarial parasites Brugia malayi and Wuchereria bancrofti [10]. In the present study we describe the cloning of a TCTP homologue from the human parasite Schistosoma mansoni. Results from the present study show that similar to the filarial TCTPs, the SmTCTP exhibit a calcium-binding property and mediate histamine release from rat basophilic leukemia (RBL-2H3) cells
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
