Cloning and characteristics of a novel geneHbUEPfrom latex inHevea brasiliensis

Abstract

AbstractHbUEP, an ubiquitin extension protein gene from latex of the rubber tree (Hevea brasiliensis) was cloned and sequenced using a differentially ethphon-induced expressed cDNA subtraction library. The cDNA had 771 bp nucleotides, comprising a 226 bp 3′ untranslated region (UTR), 77 bp 5′UTR and a 468 bp open reading frame encoding a 156 amino acid peptide. Southern blotting analysis showed that this gene was a low copy number gene in theH. brasiliensisgenome. Within 24 h after application of ethphon, the gene was expressed weakly in both control and latex sampled at 6 h, and strongly in latex sampled at 12 h, showing that this gene expression could be regulated by ethphon. Ethphon could increase the latex yield inH. brasiliensis. It is suggested that theHbUEPgene may be involved in the regulation of ethphon-induced high latex yield inH. brasiliensis.