Abstract
On the basis of conserved sequences characteristic of the Ran/TC4 subfamily of the GTPase superfamily a fragment of the gene encoding a Plasmodium falciparum Ran/TC4 homologue was amplified in the polymerase chain reaction. The fragment was used to screen a cDNA library to obtain clones which allowed determination of the complete gene sequence. The gene designated pfran ( Plasmodium falciparum ras-like nuclear protein) has around 70% amino acid identity with previously characterised Ran/TC4 proteins. Like other malarial mRNAs the pfran mRNA contains a long (at least 679 bp) 5′ untranslated region. Southern blotting experiments show that pfran is a single copy gene located on chromosome 11. RNA hybridisation experiments indicate that pfran mRNA is abundant in late trophozoite and schizont stages but present at very low levels in gametocytes and early asexual stages.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.