Abstract
Rho-related GTPase from plants (ROP) proteins play an essential role in plant stress resistance. In this study, the full-length <i>GlROP6</i> gene was cloned based on <i>G. littoralis</i> transcriptome sequencing data acquired in response to salt stress. The protein sequence, conserved domains, secondary structure, three-dimensional structure, phylogenetic relationships, and expression pattern of the <i>GlROP6</i> gene were systematically analysed. Our results showed that the full-length <i>GlROP6</i> gene had an open reading frame of 606 bp, which encoded 201 amino acid residues with a relative molecular weight of 22.23463 kDa and a theoretical isoelectric point of 9.06. Amino acid sequence analyses indicated that the structure of the GlROP6 protein was conserved, and included five G-box motifs (G1–G5), an effector binding region, a Rho insert region and a C-terminal hypervariable region. According to our phylogenetic analysis, the GlROP6 protein was closely related to the ROP protein of <i>Daucus carota</i> subsp. <i>Sativus</i>. Our quantitative real-time PCR results revealed that <i>GlROP6</i> was highly expressed in flower, and <i>GlROP6</i> expression was significantly upregulated in <i>G. littoralis</i> roots treated with NaCl. This study will facilitate investigations into the function of <i>GlROP</i> genes in response to salt stress in <i>G. littoralis</i>.
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