Abstract
Taking mixed sample of 115 d and 135 d Carya illinoinensis ‘Pawnee’ as experimental materials, after RT-PCR amplification, cloning and sequencing, the gene sequences of CiSAD gene, CiFAD2 gene and CiGPAT gene related to the key enzymes of oil metabolism in Carya illinoinensis were obtained, and bioinformatics analysis was carried out. The length of CiSAD gene was 1 240 bp, containing a complete open reading frame (ORF) of 1 194 bp, encoding 397 amino acids. CiFAD2 was 1 329 bp, containing an ORF of 1 155 bp, encoding 384 amino acids. CiGPAT was 1 671 bp, containing an ORF of 1 626 bp, encoding 541 amino acids. All proteins encoded by the cloned genes have corresponding domains, indicating that the proteins encoded by these genes should have corresponding functions in organisms.
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