Cloning and Bioinformatics Analysis of Chlorophyll Degrading Gene &lt;i&gt;PPH&lt;/i&gt; from &lt;i&gt;Curcuma alismatifolia&lt;/i&gt;

Huaqiao Ding,Jianxin Liu,Wei Hu,Qing Dong,Lihui Mao

doi:10.5376/pgt.2020.11.0003

Abstract

To obtain key enzyme gene PPH in chlorophyll degradation process of Curcuma alismatifolia , on the basis of obtaining a large amount of transcriptome information by sequencing the full-length transcriptome, we had screened and analyzed these transcriptome information and obtained 2 PPH genes which named PPH1 and PPH2 . The PPH1 gene (GenBank: MT077178) has a full-length cDNA sequence of 1 795 bp in length, an open reading frame of 1437 bp (from 138 to 1 574 bp), and encode a sequence with 478AA amino acid. The PPH2 gene (GenBank: MT077179) has a full-length cDNA sequence of 1393bp, an open reading frame of 1227bp (from 70 to 1296bp), and encode a sequence with 408AA amino acid. Using Blast, Translate tool (ExPASy), Clustal Omega, Find Conserved Domains (NCBI), ProtParam, TMHMM Server, SOPMA, SWISS-MODEL, ClustalX (1.81), MEGA4.1 and so on. Their amino acid composition, physical and chemical properties, conserved domains, secondary structures, tertiary crystal structures, and molecular phylogeny were predicted and analyzed. The nucleotide and protein amino acid sequences of PPH1 and PPH2 have high homology with these PPH genes of other species, and both of them contain a conserved region PLN02578 with hydrolase characteristic. Molecular phylogenetic analysis showed that a small cluster of PPH1 and PPH2 were closest to Musa acuminate PPH (XP_018677219.1), but far away from dicotyledons. This study provided a molecular basis for improving color of Curcuma alismatifolia sterile bracts by genetic transformation in the future.

Highlights

Curcuma alismatifolia Gagnep, called ‘Siam Tulip’ or ‘Tropical Tulip’, is a perennial bulbous herbaceous flower of Curcuma in Zingiberaceae, which is native to Chiang Mai and other places in Thailand
1.1 Acquisition of PPH1 and PPH2 From the database, 13 pieces of information annotated as pheophytinase were screened out, of which 11 pieces were 1-2K and 2 pieces were 2-3K
The results showed that PPH1 and PPH2 were clustered into a small group, and they had the closest genetic relationship with Musa acuminata subsp

Summary

Introduction

Curcuma alismatifolia Gagnep, called ‘Siam Tulip’ or ‘Tropical Tulip’, is a perennial bulbous herbaceous flower of Curcuma in Zingiberaceae, which is native to Chiang Mai and other places in Thailand. The phenomenon is very obvious when it is in the open air all day, while it is relatively light when in the shade The emergence of this phenomenon makes the bracts of Curcuma alismatifolia Gagnep show a scorching feeling, which seriously affects the beauty and reduces the ornamental value. There is no report about the key enzyme gene PPH in chlorophyll degradation and metabolism of Curcuma alismatifolia Gagnep. The purpose of this study is to obtain the key enzyme gene PPH information of chlorophyll degradation, provide a basis for improving the color of Curcuma alismatifolia Gagnep sterile bracts in the future, and pave the way for further enriching and exploring the theory of chlorophyll degradation and metabolism

Objectives

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Conclusion