Abstract
Alanine racemase activity was investigated in the industrial organismBacillus licheniformis. Two putative alanine racemase genes were identified from the genome sequence (which we denotealr andalr2) and these were cloned individually inE. coli. Expression of either of the clonedB. licheniformis genes in the alanine auxotrophic strainE. coli MB2159 complemented the mutant, restoring the ability of the recombinant host strain to grown on media such as LB without the addition of alanine. Alanine racemase activity was also directly detected in assays of cell-free extracts prepared from the recombinant strains. The results demonstrate thatB. licheniformis encodes two separate alanine racemase enzymes.
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