Abstract
ABSTRACT Galactose-binding lectins (GBLs) are a subdivision of jacalin-related lectins and known to perform an essential role in plant protective mechanisms against plant pathogens, fungi, and viruses. A cDNA sequence encoding GBL was cloned and the gene has an open reading frame (ORF) of 657 bp, encoding a protein of 219 amino acids. The protein is predicted to have a molecular weight and isoelectric point (pI) of 23.76 kDa and 6.29, respectively. GBL has a jacalin-type lectin domain and fits into the JRL superfamily. The evolutionary relationship of GBL revealed that the GBL from Morus multicaulis was firmly related to that of Morus rotundibila, Morus alba, Morus notabilis, Artocarpus integer, Artocarpus nitidus and Champedak (cgb). qRT-PCR analysis showed that the gene was expressed in all the tissues tested, leaf, and bud displaying the highest transcript levels. There was a general decrease in the mRNA transcript level under cold and salt stress as compared to the mRNA transcript level of the control. SDS-PAGE and western blot results showed that His-tagged fusion GBL protein was positively expressed in E. coli. In total, our findings disclosed the molecular basis for the signal transduction mechanisms when abiotic stress is induced in a mulberry tree.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: The Journal of Horticultural Science and Biotechnology
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
