Abstract
AbstractDNA sequence from Aspergillus oryzae genome which, confers on pOK‐1 the ability to replicate autonomously in Saccharomyces cerevisiae is described. A hybrid plasmid pOK‐1 was constructed from pBR325 and YEp13 fragment containing Leu2+ gene. Asp. oryzae DNA and pOK‐1 were cleaved with BamHI, ligated and introduced into Escherichia coli. About 600 recombinant plasmids were obtained. One of them transforms S. cerevisiae SHY3 to Leu2+ with middle efficiency. The plasmid designated as pSB1–2 contained two BamHI fragments (4.3 kb and 4.1 kb) of Asp. oryzae DNA.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
