Abstract
A modified S-SAP (sequence-specific amplified polymorphism) method with universal primers for retrotransposons was used to study <i>Vitis vinifera</i> cv. Pinot noir. Five Pinot noir clones (20Gm, 1–44Gm, 18Gm, 20–13Gm, 1–84Gm) were analyzed by 30 S-SAP primer combinations employing five <i>Mse</i>I- and six universal retrotransposon primers. Altogether 670 markers were generated, revealing 4.8% clonal variation and four out of five Pinot noir clones could be differentiated. This S-SAP method provides an efficient tool to randomly screen for polymorphisms produced through retrotransposition processes in the <i>Vitis</i> genome.
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