Abstract
Here, we determined the genes encoding antibiotic resistance enzymes and virulence factors and evaluated the genetic relationship between Enterobacter spp. isolated from different clinical samples. A total of 57 clinical isolates of Enterobacter spp. were tested for the production of extended-spectrum β-lactamases (ESBLs), carbapenemase, and AmpC using phenotypic and genotypic methods. The most common ESBLs and AmpC β-lactamases were bla TEM (63.3%) and bla EBC (57.7%), respectively. The most prevalent virulence gene was rpos (87.7%). The random amplified polymorphic DNA (RAPD) patterns of strains were genetically unrelated. RAPD polymerase chain reaction analysis revealed high genetic diversity among isolates.
Highlights
We determined the genes encoding antibiotic resistance enzymes and virulence factors and evaluated the genetic relationship between Enterobacter spp. isolated from different clinical samples
A total of 57 isolates of Enterobacter spp. were obtained from different patients admitted to three teaching hospitals of the Tehran University of Medical Sciences between September 2013 and April 2014
The phenotypic and genotypic characteristics of ESBL and AmpC-producing isolates of E. cloacae and E. aerogenes are shown in Table 2 and Table 3, respectively
Summary
Clonal relation and antimicrobial resistance pattern of extended-spectrum β-lactamase- and AmpC β-lactamaseproducing Enterobacter spp. isolated from different clinical samples in Tehran, Iran. Roya Ghanavati[1], Mohammad Emaneini[1], Davood Kalantar-Neyestanaki[2], Azin Sattari Maraji[1], Mosayyeb Dalvand[1], Reza Beigverdi[1] and Fereshteh Jabalameli[1].
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