Abstract
A protocol was developed for microporpagation of Wattakaka volubils (L.f) Stapf, an important medicinal plant in India. In vitro shoot multiplication nodal explants of W. volubilis was achieved on solid MS basal medium supplemented with BAP (6-Benzylaminopurine) and NAA (Naphthalene acetic acid). Presence of NAA in the culture medium along with BAP promoted multiplication of shoots than BAP alone. The highest shoot multiplication rate (23.4±0.48) was observed after 28 days of culture on MS basal medium supplemented with 0.6 mg/l BAP and 0.2 mg/l NAA. Elongated shoots produced roots within 6 weeks in half strength MS basal medium supplemented with 0.6 mg/l IBA (Indole-3-butyric acid) and 3% (w/v) sucrose. Rooted plantlets were acclimatized with a survival rate of 95% and transferred to the green-house and thereafter successfully transferred to the field with 90% survival rate. Keywords: medicinal plants, micropropagation, nodal culture, survival rate DOI: http://dx.doi.org/10.4038/cjsbs.v40i1.3406 CJSBS 2011; 40(1): 53-58
Highlights
Medicinal plants are an important health care and economic resource
For root induction, separated shoots were transferred to half strength MS basal medium supplemented with different concentrations of IBA 0.2-1.2 mg/l, IAA 0.2-1.2 mg/l and Naphthalene acetic acid (NAA) 0.1-0.6 mg/l and 2% (w/v) sucrose
Multiple shoots and buds originated from nodal explants, when MS medium was supplemented with different concentrations and combinations of BAP (0.2-1.2 mg/l), KIN (0.21.2 mg/l), NAA (0.2-1.2 mg/l) and GA3 (0.2-1.2 mg/l)
Summary
Medicinal plants are an important health care and economic resource. India is one of the world’s richest countries in medicinal plants comprising about 45000 species. To raise stock culture, nodal explants were taken from the green house plants. Well developed 3 year - old mature plants were cut into 2-3 cm nodal segments and used for induction of multiple shoots.
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