Abstract
To investigate the genetic background for the emergence of macrolide resistance, we characterized the genetic features of Mycoplasma pneumoniae using multilocus sequence typing. Of the 146 M. pneumoniae strains collected during the 5 consecutive outbreaks of M. pneumoniae pneumonia during 2000–2016 in South Korea, macrolide resistance increased from 0% in the first outbreak to 84.4% in the fifth. Among the 8 sequence types (STs) identified, ST3 (74.7%) was the most prevalent, followed by ST14 (15.1%). Macrolide-susceptible strains comprised 8 different STs, and all macrolide-resistant strains were ST3 (98.3%) except 1 with ST14. The proportion of macrolide-resistant strains in ST3 remained 2.2% (1/46) until the 2006–2007 outbreak and then markedly increased to 82.6% (19/23) during the 2010–2012 outbreak and 95.0% (38/40) during the 2014–2016 outbreak. The findings demonstrated that clonal expansion of ST3 M. pneumoniae was associated with the increase in macrolide resistance in South Korea.
Highlights
Joon Kee Lee,1 Joon Ho Lee,1 Hyunju Lee, Young Min Ahn, Byung Wook Eun, Eun Young Cho, Hwa Jin Cho, Ki Wook Yun, Hoan Jong Lee, Eun Hwa Choi
Until the 2006–2007 outbreak, DNA samples were directly collected from respiratory samples (64 samples), and DNA samples from the 2010–2012 outbreak were extracted from cultured M. pneumoniae (82 samples)
In this study, molecular microbiological analysis of multilocus sequence typing (MLST) found that the increase of macrolide resistance in South Korea during a 17-year period (2000–2016) was related to changes in genetic backgrounds of M. pneumoniae strains
Summary
Joon Kee Lee, Joon Ho Lee, Hyunju Lee, Young Min Ahn, Byung Wook Eun, Eun Young Cho, Hwa Jin Cho, Ki Wook Yun, Hoan Jong Lee, Eun Hwa Choi. Of the 146 M. pneumoniae strains collected during the 5 consecutive outbreaks of M. pneumoniae pneumonia during 2000–2016 in South Korea, macrolide resistance increased from 0% in the first outbreak to 84.4% in the fifth. The findings demonstrated that clonal expansion of ST3 M. pneumoniae was associated with the increase in macrolide resistance in South Korea. Since the first report of a macrolide-resistant M. pneumoniae isolated from a child in Japan in 2001 [12], several countries in Asia, including South Korea, Japan, and China, have reported increased prevalence of macrolide resistance [13,14,15,16]. To clarify the genetic diversity of M. pneumoniae strains between outbreaks, we conducted an MLST analysis of M. pneumoniae detected from children in whom community-acquired pneumonia was diagnosed during 5 consecutive epidemics of M. pneumoniae pneumonia during 2000–2016 in South Korea. We sought to find the genetic background that may explain the emergence of macrolide resistance among M. pneumoniae strains
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
