Abstract
To disclose the antibiotics susceptibility and wide adaptability of commonly occurring genotypes of Salmonella Typhimurium, the antibiotic resistance and biofilm formation of different multi-locus sequence typing (MLST) types of a collection of 240 S. Typhimurium isolates (33 food and 207 clinical ones) during 2010–2014 in Shenzhen were analyzed. Among these strains, 167 was ST34 (69.58%), and 57 was ST19 (23.75%), respectively. A total of 159 (95.21%) ST34 strains displayed the multidrug resistant phenotype (≥ three classes of antibiotic), whereas only 23 (40.35%) ST19 ones did (P < 0.01). Moreover, a relative high proportion (72.46%) of ST34 isolates was classified as moderate to strong biofilm-producers, while only 15.79% of ST19 (P < 0.01) was. Among the food isolates, more than half (51.52%) were from livestock products, among which 41.18% classified as moderate to strong biofilm-producers. In summary, this study highlights the expansion of S. Typhimurium ST34 of strong biofilm-forming ability and multidrug resistance in the southern coastal region of China. Therefore, monitoring the occurrence of ST34 S. Typhimurium in food sources, especially in livestock products, and taking appropriate measures to control Salmonella spp. infections via decreasing biofilm formation should be addressed.
Highlights
The genus Salmonella is a major causative agent of foodborne illnesses, responsible for more than 90 million cases of salmonellosis annually across the globe (Majowicz et al, 2010; Havelaar et al, 2015)
Typhimurium isolates obtained from food and clinical sources will help us in understanding their adaptability, so as to identify the potential risk of Salmonella infection
Typhimurium isolates, 207 strains with 168 different PFGE patterns and other 33 food isolates recovered from product of poultry or livestock, ready to eat food, and aquatic products (Table S1) were selected for this study, The stock cultures were maintained at −80◦C in tryptone soy broth (TSB; Oxoid Ltd., Cambridge, U.K.) supplemented with 20% glycerol
Summary
The genus Salmonella is a major causative agent of foodborne illnesses, responsible for more than 90 million cases of salmonellosis annually across the globe (Majowicz et al, 2010; Havelaar et al, 2015). In China, more than 75% of Salmonella spp. isolates studied are resistant to at least one antibiotic It has been reported that the ability to form biofilms by foodborne pathogens was closely related to human illness and the increased risk of food safety (Steenackers et al, 2012). Typhimurium isolates obtained from food and clinical sources will help us in understanding their adaptability, so as to identify the potential risk of Salmonella infection. Typhimurium isolates collected during 2010–2014, including 207 clinical isolates and 33 food ones, to understand the adaptability of the most common genotypes, so as to provide measures for controlling the prevalence of S.
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