Abstract
As the second leading etiological agent of candidemia in Turkey and the cause of severe fluconazole-non-susceptible (FNS) clonal outbreaks, Candida parapsilosis emerged as a major health threat at Ege University Hospital (EUH). Evaluation of microbiological and pertinent clinical profiles of candidemia patients due to C. parapsilosis in EUH in 2019–2020. Candida parapsilosis isolates were collected from blood samples and identified by sequencing internal transcribed spacer ribosomal DNA. Antifungal susceptibility testing was performed in accordance with CLSI M60 protocol and ERG11 and HS1/HS2-FKS1 were sequenced to explore the fluconazole and echinocandin resistance, respectively. Isolates were typed using a multilocus microsatellite typing assay. Relevant clinical data were obtained for patients recruited in the current study. FNS C. parapsilosis isolates were recovered from 53% of the patients admitted to EUH in 2019–2020. Y132F was the most frequent mutation in Erg11. All patients infected with C. parapsilosis isolates carrying Y132F, who received fluconazole showed therapeutic failure and significantly had a higher mortality than those infected with other FNS and susceptible isolates (50% vs. 16.1%). All isolates carrying Y132F grouped into one major cluster and mainly recovered from patients admitted to chest diseases and pediatric surgery wards. The unprecedented increase in the number of Y132F C. parapsilosis, which corresponded with increased rates of fluconazole therapeutic failure and mortality, is worrisome and highlights the urgency for strict infection control strategies, antifungal stewardship, and environmental screening in EUH.
Highlights
Candidemia represents a major global public health concern due to high mortality rates and additional economic burden (Arastehfar et al, 2020f)
Continuous monitoring of the burden of antifungal resistance as well as analysis of the underlying molecular mechanisms and genotypic diversity of the isolates in relation to clinical data are of FIGURE 2 | 30-day survival of patients infected with azole-susceptible and azole-resistant C. parapsilosis isolates carrying Y132F in Erg11
An alarming finding is that approximately 53% of patients with candidemia in this study were infected with FNS C. parapsilosis and all of them showed fluconazole therapeutic failure (FTF) when treated with azoles
Summary
Candidemia represents a major global public health concern due to high mortality rates and additional economic burden (Arastehfar et al, 2020f). Global epidemiological studies characterize Candida albicans as the leading cause of candidemia (Pfaller et al, 2019), non-albicans Candida (NAC) species, especially C. parapsilosis, C. tropicalis, C. glabrata, and C. auris, surpass C. albicans in some countries and individual patient population and healthcare institutions (Arastehfar et al, 2020f; Megri et al, 2020). Among these NAC species, C. parapsilosis stands out as an important causative agent of invasive candidiasis observed in the last decade (Pfaller et al, 2019). It is highly important to diligently monitor the burden of antifungal resistance and perform genotyping of C. parapsilosis blood isolates in clinical settings that have experienced fungal infection outbreaks
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
