Clonal analysis of vertebrate myogenesis: VIII. Fibroblast growth factor (FGF)-dependent and FGF-independent muscle colony types during chick wing development

Abstract

The effect of bovine fibroblast growth factor (FGF) on the in vitro differentiation of various stage-specific populations of skeletal muscle colony-forming (MCF) cells from the developing chick wing bud was examined. The results show that bovine FGF (3 ng/ml daily) delays the onset of differentiation of MCF cells obtained from Day 4–12 wing buds by about 1 day; but, in addition, the results demonstrate that a subset of colony-forming cells derived from stage 23–27 (Day 4–5) embryos require FGF for myogenic differentiation. The FGF-dependent MCF cells attach and grow in the absence of FGF, but do not differentiate unless given FGF within 1–3 days after inoculation. Thus, between stages 23 and 27 the myogenic population contains discrete subclasses that are FGF dependent and others that are FGF independent. Both subclasses are found within two of the previously classified MCF cell populations, the early and late MCF cells. FGF-dependent and independent early MCF cells are present within the wing bud until stage 25, after which only the FGF-independent early MCF subclass persists. Similarly, both FGF-dependent and -independent late MCF cells are present between stages 25 and 27, but only the FGF-independent late MCF subclass remains after stage 31. The mechanisms responsible for relative changes in the proportions of MCF cell subclasses and for the FGF requirements are not understood. In addition, while FGF is required, there is no evidence suggesting that FGF triggers skeletal muscle terminal differentiation within the FGF-dependent MCF cell subclasses.