Abstract
Hematopoietic stem cells (HSCs) are defined as cells present in the bone marrow that are capable of both self-renewal and multilineage differentiation. The differentiation pathway from HSCs to mature blood cells and HSC self-renewal and differentiation mechanisms still remain unclear and require further elucidation. However, HSCs are a highly diverse population with analysis limitations at the population level. Moreover, molecular and cell biological single-cell analysis has been attracting tremendous attention recently. Herein, we introduce a cell biological single-cell analysis method (single-cell transplantation and cell lineage tracing experiments using genetic techniques) in the field of HSCs.
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