Clitoria ternatea root extract improves cognitive impairment and cholinergic dysfunction in animal model of chronic cerebral hypoperfusion

Abstract

Event Abstract Back to Event Clitoria ternatea root extract improves cognitive impairment and cholinergic dysfunction in animal model of chronic cerebral hypoperfusion Thenmoly Damodaran1, Sharif Mahsufi Mansor1, Lim G. Keat2, Vikneswaran Murugaiyah3, Surash Ramanathan1 and Zurina Hassan1* 1 Universiti Sains Malaysia, Centre for Drug Research, Malaysia 2 Universiti Sains Malaysia, School of Chemical Sciences, Malaysia 3 Universiti Sains Malaysia, School of Pharmaceutical Sciences, Malaysia Clitoria ternatea (CT) commonly known as butterfly pea has been used to promote brain function and to treat mental disorders in Indian Ayurvedic medicine. Chronic cerebral hypoperfusion (CCH) has been considered as the major cause of cognitive impairment that occurs in human aging and vascular dementia. This study aimed to investigate the effects of the methanolic extract of Clitoria ternatea root (CTR) on cognitive impairment induced by CCH in rats and to propose cholinergic system alteration as one of the mechanism of action. Male Sprague Dawley rats (200-300g) were subjected to permanent bilateral occlusion of common carotid arteries (PBOCCA) or sham-operated surgery. These rats were then given oral administration of CTR at doses of 100, 200 and 300 mg/kg, respectively for 28 days. Passive avoidance task and Morris water maze paradigms were used to evaluate the learning and memory performances. At the end of experiment, brain was removed and the acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE) activities were measured in prefrontal cortex, hippocampus and neocortex. The methanolic extract of CTR at the dose of 200 and 300 mg/kg significantly reversed the memory impairment induced by CCH in the passive avoidance task and reduced the escape latencies during training in Morris water maze, as well as increased the percentage of time spend in the target quadrant of the maze. In addition, the increased AChE activity in prefrontal cortex and hippocampus produced by CCH was significantly inhibited by CTR at high dose. However, there was no significant changes observed in BuChE activity between groups for all regions. The results suggested that CTR could be a potential protective agent against CCH and to improve the cholinergic function which may contribute to the improving effect of CTR on learning and memory. However, further studies are needed to explore the mechanism of action of CTR. Acknowledgements Financial support was received from Ministry of Higher Education (MOHE) Malaysia and Universiti Sains Malaysia special funding for the project of Fundamental Neuroscience-Neurobehaviour (304/CDADAH/652201/K134) and RUI (1001/CDADAH/812177) Keywords: Cognition, in vivo, natural product, Cholinergic function, Behavioural test Conference: 14th Meeting of the Asian-Pacific Society for Neurochemistry, Kuala Lumpur, Malaysia, 27 Aug - 30 Aug, 2016. Presentation Type: Poster Presentation Session Topic: 14th Meeting of the Asian-Pacific Society for Neurochemistry Citation: Damodaran T, Mahsufi Mansor S, Keat LG, Murugaiyah V, Ramanathan S and Hassan Z (2016). Clitoria ternatea root extract improves cognitive impairment and cholinergic dysfunction in animal model of chronic cerebral hypoperfusion. Conference Abstract: 14th Meeting of the Asian-Pacific Society for Neurochemistry. doi: 10.3389/conf.fncel.2016.36.00109 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 04 Aug 2016; Published Online: 11 Aug 2016. * Correspondence: Dr. Zurina Hassan, Universiti Sains Malaysia, Centre for Drug Research, Georgetown, Penang, Malaysia, Zurina.Hassan@frontiersin.org Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Thenmoly Damodaran Sharif Mahsufi Mansor Lim G Keat Vikneswaran Murugaiyah Surash Ramanathan Zurina Hassan Google Thenmoly Damodaran Sharif Mahsufi Mansor Lim G Keat Vikneswaran Murugaiyah Surash Ramanathan Zurina Hassan Google Scholar Thenmoly Damodaran Sharif Mahsufi Mansor Lim G Keat Vikneswaran Murugaiyah Surash Ramanathan Zurina Hassan PubMed Thenmoly Damodaran Sharif Mahsufi Mansor Lim G Keat Vikneswaran Murugaiyah Surash Ramanathan Zurina Hassan Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. 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