Abstract
Objective:To study the clinocopathological factors and presence of Human Pappiloma Virus in ameloblastoma by immnohistochemistry.Methods:It was a cross sectional study on 50 surgical specimens of ameloblastoma, completed in six months. These were selected and processed for initial screening by H&E and then by immunohistochemistry (IHC) for detection of Human Papilloma Virus (HPV). The questionnaire was designed to study the clinicopathological factors associated in these patients. Sections of 4µm were cut, placed on special positive charged glass slides in the Department of Pathology, King Edward Medical University. It was then examined by the histopathologists for grading and scoring of these lesions. Chi Square test was used to assess the differences found in types of ameloblastomas. The p-value was smaller than 0.05 (p < 0.05).Results:The mean age of the patients (12-80 years old) was 38.6±15.1 years, with male-female ratio 2.84: 1. HPV was positive in 9 (18%), whereas negative in of 41 (82%) patients. Among the positive, reactive HPV with score-1 was 8 and score-2 was 1. According to histological variant, follicular was present in 78%, Plexiform pattern in 8%, Conventional and Desmoplastic variants in one patient each; and Cystic and Acanthomatous were seen in two and three patients respectively. The mandible was involved in 39 patients, maxilla and right maxilla involved in 4 patients each, right retromolar, cheek and angle of mandible was seen in one patient each. About 16% patients had anterior, 66% had posterior and 18% had both anterior and posterior regions involved. Among the HPV positive reactive statistically, no significant difference was found with smoking, Paan and exposure to pesticides, factory or mine (p-value > 0.05). Among HPV positive reactive patients, eight had ameloblastoma whereas, 1 had ameloblastomic fibroma. There was no statistical significance of type, location and region of tumor in HPV positivity.Conclusion:Mandible and posterior region was more commonly involved. Follicular pattern was most common. There was no effect of exposure to pesticides, factory or mine, smoke and human papilloma virus in the etiology of ameloblastoma because only 18% of patients showed the association of HPV16
Highlights
Odontogenic tumours (OT) are benign tumors of the dental tissue arising from the mandible, the maxilla rarely from the gingiva
The purpose of this study was to investigate the typical histological changes caused by human papilloma virus in ameloblastoma by immunohistochemistry
Human Papilloma Virus (HPV) positivity was detected in few cases of ameloblastomas HPV positivity was observed more in the follicular cases than another type
Summary
Odontogenic tumours (OT) are benign tumors of the dental tissue arising from the mandible, the maxilla rarely from the gingiva. Broca first described an odontogenic neoplasm in 1868 and various classifications have been proposed.[1] Ameloblastoma is the most common OT, approximately 1% of all oral tumours and affecting young adults in the fourth and fifth decades.[2,3] As there was little. Peripheral ameloblastoma and peripheral intraosseous ameloblastoma are two clinical variants of ameloblastoma, which present as an exophytic soft-tissue lesion.[5] The pathogenesis of such tumors is still unclear. There are evidences of association of HPV with the development of benign and premalignant lesions, and malignant tumours. It has been found in healthy mucosa as well. HPV dominance in oral lesions, including oral cancers, has been shown to vary from 0% to 100%.6
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