CLINICOPATHOLOGICAL HEMETOLOGICAL AND MOLECULAR STUDY OF DENGUE SUSPECTED PATEINTS -A PROSPECTIVE STUDY IN TERTIARY CARE HOSPITAL

Abstract

Background: Dengue is common mosquito born viral disease of human caused by dengue virus .Dengue virus belongs to family of Flaviviridae and there are 4 serotypes of virus which are DENV-1 to DENV -4. Across the world about 100 million infections are detected every year in which 5,00,000 manifest in hemorrhagic cases.By clinical presentation and laboratory test, infection with dengue can be diagnosed. The study was from March 2020 to NovemberMethod: 2021.Total 200 samples were taken .All the patients suspected for dengue were considered in this study and after ELISA only positive cases for NS1 were undertaken for molecular study. A total 200 clinically suspected samples wereResult: taken .Out of 200 samples 120 (60.0%) were dengue positive ,80 (40.0%) were dengue negative .Out of 120 serologically dengue positive samples, 81 (67.5%) were positive for NS1 antigen and 39 were positive for IgM by ELISA. Out of 120 dengue posistive cases fever was the most common clinical presentation in 120 (100%) and thrombocytopenia was the most common hematological finding present in 102 (85.0 %) cases . Out of 81 (67.5%) dengue NS1 antigen positive samples on which molecular study was done showed, 22 (27.16%) positive for dengue virus RNA and 59 (72.84%) negative for dengue virus RNA by conventional PCR method. Awareness of clinical features, laboratoryConclusion: findings consisting of hematological and serological parameters as well as molecular study are important for diagnosis and management of dengue and dengue like illness.