Clinicopathological Evaluation of Anti-inflammatory and Hepatoprotective Effects of Rutin in Guinea pigs

Abstract

This study was carried out to evaluate the anti-inflammatory activity of flavonoid (Rutin 20 mg/kg) on each (carrageenan-induced paw edema)acute inflammation and (cotton pellet granuloma) chronic inflammation and, effect of Rutin on some hematological, biochemical and histopathological examination in guinea pigs induced by inflammation. A total of eighteen male guinea pigs were categorized into three groups, six pigs on all group. Group one as non-treated control, they given 5% carboxymethyl cellulose orally for seven days. Group two received single dose orally (20mg/kg) Rutin for seven days. Group three received (10mg/kg) Indomethacin orally for seven days. By injecting of 0.1ml of carrageenan suspension in saline into the sub-planter superficies of the right hind paw, the acute inflammation (edema) was induced in all experiment guinea pigs one hour after compounds taken. Then estimated the volume of paw edema immediately after carrageenan injection through one, two, three and four hours by using a plethysmograph system(VGO Basile, Italy). The results indicated that, Rutin (20 mg/kg body weight) showed significant lowered (P<0.05) in the carrageenan induced paw edema and cotton pellet induced granuloma model comparing with control and indomethacin groups. In addition Rutin (20 mg/kg) improved the hematological and biochemical profiles and able of maintain the biochemical varies related to inflammation. This was also supported by histopathological studies of liver sections. On conclusion, Rutin possess anti-inflammatory activity. Thus the Rutin showed protective role against acute and chronic inflammation.