Abstract
We investigated the clinicopathologic significance of HIF-1, CXCR4, and VEGF expression using immumohistochemistry in human colon cancer. HIF-1, CXCR4, and VEGF high expression levels were correlated positively with TNM stage, lymph node involvement, and distant metastasis Furthermore, we found that combined high expression of any two of the three molecules (P = .028 for HIF-1/CXCR4, P = .007 for HIF-1/VEGF, and P = .004 for CXCR4/VEGF) had stronger correlation with lymph node metastasis than did each alone. However, a relationship with distant metastasis is seen only with the combinations CXCR4/VEGF (P = .069 for HIF-1/CXCR4, P = .062 for HIF-1/VEGF, and P = .035 for CXCR4/VEGF) as compared with those of single molecule high expression alone. Combined expression of all three molecules strongly correlates with lymph node metastasis and distant metastasis. The mRNA expression of HIF-1, CXCR4, and VEGF were quantified by real-time PCR in different colon cancer tissue samples, the experiment results shown that fresh colon tissue samples significantly overexpressed CXCR4 and VEGF mRNA compared with negative control. Therefore, the disease-free survival of all patients after curative resection can be considered in association with all three markers expression.
Highlights
Colon cancer is one of the most common cancers frequently metastasizing to the liver, lymph nodes, and peritoneum [1].Currently, radical surgery represents the standard method of therapy
We investigate whether the expression of HIF-1α, CXC chemokine receptor 4 (CXCR4), and Vascular endothelial growth factor (VEGF) have a significant correlation with clinicopathologic factors of colon cancer
In order to quantify the message RNA of these marks in colon cancer, expression of HIF-1α, CXCR4, and VEGF at the mRNA levels was analyzed by real-time PCR in different samples
Summary
Colon cancer is one of the most common cancers frequently metastasizing to the liver, lymph nodes, and peritoneum [1]. CXCR4 is the most common chemokine expressed in human tumors such as breast cancer, colorectal cancer, and ovarian cancer, and SDF-1 is highly expressed at sites of metastasis including the lung, bone marrow, lymph nodes, and liver [10]. The prognostic value of overexpression of VEGF has been demonstrated in many types of solid human cancers Based on these studies, we suggest that combinations of HIF-1α, CXCR4, and VEGF expression in tumor tissue will be useful for predicting clinicopathologic significance and tumor metastasis. We investigate whether the expression of HIF-1α, CXCR4, and VEGF have a significant correlation with clinicopathologic factors of colon cancer. The mRNA expression of HIF-1α, CXCR4, and VEGF in colon cancer were quantified by real-time PCR
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