Abstract
BackgroundThe goal of this study was to investigate the diagnostic value of miR-29c-3p in sepsis and its role in sepsis-induced inflammatory response and cardiac dysfunction.MethodsSerum level of miR-29c-3p was detected by qRT-PCR. The ROC curve was used to evaluate the diagnostic value of miR-29c-3p for Sepsis. The cecal ligation and puncture method (CLP) was used to establish a rat sepsis model. To assess cardiac function, left ventricular systolic pressure (LVSP), left ventricular end diastolic pressure (LVEDP) and maximum rate of rise/fall of left ventricle pressure (± dp/dtmax) in different experimental groups were detected, and the serum cardiac troponin I (cTnI), creative kinase isoenzyme MB (CK-MB) were measured by ELISA. Meanwhile, TNF-α, IL-1β, and IL-6 were detected by ELISA to assess the level of inflammatory response in animals.ResultsmiR-29c-3p level was upregulated in sepsis patients. ROC curve revealed that miR-29c-3p had the ability to distinguish sepsis patients from healthy controls. Cardiac dysfunction and inflammation were observed in sepsis rat, which were characterized by the decrease of LVSP and + dp/dtmax, the increase of LVEDP, − dp/dtmax, cTnI, CK-MB, TNF-α, IL-1β, IL-6. All effects were reversed by the injection of miR-29c-3p antagomir. Logistics regression analysis manifested miR-29c-3p is an independent factor in the occurrence of cardiac dysfunction in sepsis patients.ConclusionsmiR-29c-3p has potential as a biomarker for the diagnosis of sepsis, and inhibition of miR-29c-3p expression in animal models reduced sepsis-induced cardiac dysfunction and inflammatory response.
Highlights
The goal of this study was to investigate the diagnostic value of miR-29c-3p in sepsis and its role in sepsis-induced inflammatory response and cardiac dysfunction
Participants and samples The study has been authorized by the Ethics Committee of Gongli Hospital of Pudong New Area of Shanghai, and each participant and his/her family members have been informed and signed a written informed consent. 86 sepsis patients admitted to the intensive care unit (ICU) of this hospital were recruited and the diagnostic criteria for sepsis follow the international diagnostic criteria for Sepsis 3.0 issued by SCCM/ESICM in 2016 [12]
Serum level of miR‐29c‐3p in sepsis patients qTR-PCR results showed that serum miR-29c-3p level in sepsis patients was significantly upregulated in comparison to healthy controls (Fig. 1A, P < 0.001)
Summary
The goal of this study was to investigate the diagnostic value of miR-29c-3p in sepsis and its role in sepsis-induced inflammatory response and cardiac dysfunction. Sepsis 3.0 defines sepsis as MiRNAs are a class of non-coding RNAs (ncRNAs) with gene regulation function and approximately 19–24 nucleotides in length [6, 7]. In the myocardial cells of the sepsis mouse model, miR-223 expression was significantly down-regulated, and the further deletion of miR-223 aggravated the cardiac insufficiency and inflammatory response of the sepsis mice [9]. A recent study showed abnormally high miR-29c-3p in inflammatory diseases, such as adult-onset Still’ s disease (AOSD) patients [11]. Considering that there are relatively few studies on the relationship between miR-29c-3p and sepsis, the specific mechanism remains to be explored
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