Clinical Validity of Tumor-Informed Circulating Tumor DNA Analysis in Patients Undergoing Surgery of Colorectal Metastases.

Abstract

Accurate biomarkers to monitor tumor load and response in metastatic colorectal cancer patients undergoing surgery could optimize treatment regimens. This study explores the clinical validity of tumor-informed quantification of circulating tumor DNA in blood using ultra-deep sequencing. Resection specimens from 53 colorectal cancer patients were analyzed for tumor-specific mutations in 15 genes. These mutations were used to measure the presence of circulating tumor DNA in preoperatively collected plasma samples using hybrid capture-based sequencing. Additional postoperative measurements were performed one week after surgery in sixteen patients. The study was conducted at the Radboud university medical center. A total of 53 colorectal cancer patients undergoing surgery of metastases were included. The detection of circulating tumor DNA. At least one tumor-specific mutation was detected in all tumor samples. In preoperative plasma samples circulating tumor DNA was detected in 88% (37/42) of systemic treatment-naïve patients and in 55% (6/11) of patients who received preoperative chemotherapy. More specifically, in 0% (0/3) of cases with a subtotal or partial pathological response and in 75% (6/8) of cases without a pathological response in the resection specimen (p = 0.06). In postoperative plasma samples circulating tumor DNA was detected in 80% (4/5) of patients with an incomplete resection and in 0% (0/11) of those with a complete resection (p = 0.003). The study was limited by the heterogeneity of the cohort and the small number of postoperative plasma samples. These data indicate that tumor-informed circulating tumor DNA detection in plasma of patients undergoing surgery for metastatic colorectal cancer is feasible and may have clinical value in response monitoring and predicting residual disease. Prospective studies are needed to establish the clinical utility of circulating tumor DNA analysis to guide treatment decisions in these patients. See Video Abstract at http://links.lww.com/DCR/B990.