Abstract
Background & Objective:Concentration of low-density lipoprotein (LDL) is a known risk factor for cardiovascular disease which is routinely measured or calculated as LDL-C in clinical laboratories. In order to decrease the cost, instead of its measuring, it is recommended to calculate it using multiple formulas that have been introduced up to now. The aim of this study was to assess the results of various formulas and comparison of these results with those of measuring method and to clarify the best formula for the Iranian population.Methods:Concentrations of total cholesterol (TC), triglyceride (TG), cholesterol of high-density lipoprotein (HDL-C) and LDL-C in serums of 471 overnight fasting individuals were measured and also LDL-Cs of these samples were calculated by eleven different formulas according to their TC, TG, and HDL-C concentrations. Subsequently, results of measured and calculated LDL-C were analyzed statistically by paired t-test, correlation coefficient, and Passing-Bablok regression. In addition, for clinical evaluation, the differences between calculated and measured mean results were calculated and compared with an allowable total error.Results:Paired t-test unraveled a significant difference between the results of measured and calculated LDL-C by various formulas. But for some formulas, these differences were not clinically significant. The best clinical and statistical agreement (correlation coefficient) was obtained by the Friedewald equation.Conclusion:By using validated methods which have correct calibration and control system for measuring TC, TG, and HDL-C, we can use the Friedewald formula for calculating LDL-C in serum samples with TG up to 400 mg/dL.
Highlights
Cardiovascular disease is the most common cause of morbidity and mortality worldwide [1]
Concentrations of total cholesterol (TC), triglyceride (TG), cholesterol of high-density lipoprotein (HDL-C) and Low density lipoprotein cholesterol (LDL-C) in serums of 471 overnight fasting individuals were measured and low density lipoprotein (LDL)-Cs of these samples were calculated by eleven different formulas according to their TC, TG, and High density lipoprotein cholesterol (HDL-C) concentrations
Some clinical laboratories measure cholesterol component of LDL (LDL-C) as an estimate of LDL concentration. These homogenous direct LDL-C methods rely on measuring cholesterol of LDL particles in the presence of other lipoprotein particles which have been prevented from participating in measuring cholesterol reaction
Summary
Cardiovascular disease is the most common cause of morbidity and mortality worldwide [1]. Increased plasma low density lipoprotein (LDL) concentration is an important known risk factor for this disease [2,3,4]. One of the main goals in preventing cardiovascular disease is to reduce plasma or serum LDL concentration [1,3]. Some clinical laboratories measure cholesterol component of LDL (LDL-C) as an estimate of LDL concentration These homogenous direct LDL-C methods rely on measuring cholesterol of LDL particles in the presence of other lipoprotein particles which have been prevented from participating in measuring cholesterol reaction. Concentration of low-density lipoprotein (LDL) is a known risk factor for cardiovascular disease which is routinely measured or calculated as LDLC in clinical laboratories. The aim of this study was to assess the results of various formulas and comparison of these results with those of measuring method and to clarify the best formula for the Iranian population
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