Abstract
formation in cells derived from newborn rat calvariae (De Oliveira et al, 2007). Based on this, the aim of our study was to analyze the gene expression of key bone markers during differentiation of human mesenchymal stem cells (MSCs) into osteoblasts grown on Ti with nanotopography. Machined Ti discs, 12 mm in diameter, were chemically treated with H2SO4/H2O2 for four hours. Untreated discs were used as control. Human MSCs were obtained from bone marrow of iliac crest under the rules of the Committee of Ethics in Human Research and expanded in growth media until subconfluence. Cells were then subcultured in an osteogenic medium (2x10cells/disc) on both nanostructured and control Ti discs for periods of up to 17 days. At 4, 10 and 17 days the gene expression of key bone markers was analyzed by real-time PCR. The obtained data were compared by Mann-Withney U-test, for independent samples (n=3). Nanostructured Ti increased (p#0.05) gene expression of alkaline phosphatase (Alp) at day 4, type I collagen (Col) and osteopontin (Opn) at day 10 (p#0.05) and Runt-related transcription factor 2 (Runx2), osteocalcin (Oc), Opn and bone morphogenetic protein 4 (Bmp-4) at day 17. In conclusion, our results showed that nanotopography upregulates the gene expression of key bone markers during distinct phases of the osteoblast differentiation of human MSCs compared with untreated discs, suggesting that this surface modification may favor the osseointegration of Ti implants.
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